Evaluation of DNA damage in Cyprinus carpio (L. 1758) exposedto malathion using Single Cell Gel Electrophoresis

Authors

A. Mashinchian Moradi Department of Marine Biology, Graduate School of Marine Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran

H. Mozdarani Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

M. Alidoust Salimi Department of Marine Biology, Graduate School of Marine Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran

P. Alidoust Salimi Department of Marine Biology, Graduate School of Marine Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract:

Malathion (S-(1, 2-dicarboethoxyethyl) O, O-dimethyl phosphorodithioate) is one of the organophosphate pesticides comprehensively used in agriculture fields throughout the world. In spite of widely used, little information are available about DNA damage in aquatic organisms. Therefore, the present study carried out to investigation of DNA damage induced by malathion in Cyprinus carpio (Pisces: Cyprinidae) using single cell gel electrophoresis. The condition of experiment was determined in static system. The specimens were exposed to different non-lethal concentrations (0.5, 1.5 and 3 mgL-1) of the malathion for 96 hour (short-time exposed). Our results showed, the specimens exposed to different concentrations of malathion exhibited significantly higher DNA damage in their blood cells than the control sample (P

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Journal title

International Journal of MArine Science and Engineering

volume 2 issue 3

pages 185- 188

publication date 2012-09-01

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Keywords

Comet assay fish Genetic Damage Index (GDI) Genotoxicity Insecticides

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